Abstract:Based on previous studies, a proposal is put forward to carry out immobilization culture experiment of Thiobacillus ferrooxidans by adopting 50% inoculation rate and reducing inoculation rate successively. The test results show that this method can complete immobilization of bacteria within 10 days after inoculation for 9 times, and average oxidation rate of ferrous iron can reach 0.31-0.61 g/(L?h) after complete immobilization. Under the same conditions, average oxidation rate of ferrous iron by immobilized cells is 5 times as high as that of free cells with 20% inoculation rate and 4 times as high as that with 40% inoculation rate. At continuous culture stage, ferrous oxidation rate of highly immobilized bacteria with different materials and different columns can reach 6.67 g/(L?h). The carrier column with height of 55 cm and filled with zeolite which can be selected as subsequent column leaching test to culture active bacteria solution.