Abstract:The effects of aeration quantity and ferrous supplement on ferrous oxidized rate of acidophilic mixed culture were investigated. The results show that the acidophilic consortium grows slowly when the aeration quantity is low, while it grows faster and the memberane formation on the plastic fillings forms better when aeration quantity increases. However, excessive aeration quantity would limit the membrane formation and lower down the bacterium density. The optimum aeration quantity for 5 liters culture system is 0.6 L/min in this research. A relative higher aeration quantity could maintain a higher dissolved oxygen concentration and its equilibrium, resulting in a higher ferrous oxidation rate. The adding of ferrous to the culture could make the acidophilic consortium stay in logarithmic phase or stable phase, which is beneficial to enhance bacterium density and ferrous oxidation rate. The highest ferrous oxidation rate is 1.75 g/(L?h), 10 times more than that of the traditional batch culturing.