Abstract:Regarding Yongping copper ore as an object of study, the researches of domestication of leaching-ore bacteria have been conducted through gradually simulating the ore environment in some proportion of ore culture to artificial culture in this paper. The results show that after the bacteria of S_(2-2)+9K and S_(2-2)+9K+S two samples are domesticated four times, the inversion rate of Fe~(2+) in solution obviously improves, the precipitation rate of Fe obviously reduces, and while the pH value gradually descends. According to the analysis of adjusting the sour times, range and the degree of pH value descent, the ore-bearing 9K+S cultures more benefit to domesticate the Ferrobacillus. While according to the analysis of the inversion rate of Fe~(2+) and the precipitation rate of Fe in solution, the ore-bearing 9K cultures more benefit to domesticate the Thiobacillus. The acidity must be controlled well, and otherwise would result in the precipitation of large quantity Fe in the course of gradually domestication bacteria.